Document Type
Article
Publication Title
Journal of Bacteriology
Abstract
A competitive (nonmetabolizable) inhibitor of glucose uptake, α- methylglucoside, was used to limit the growth of Escherichia coli. Cell division during such a nutritional shift-down was studied in batch cultures and with the 'baby-machine' technique. Following a brief delay, the rate of division was maintained for 60 to 70 min in batch cultures and for an extended period in the baby machine. Decreases in cell size were due, in part, to a possible reduction in the mass per chromosome origin at the time of replication initiation and a shorter time interval between initiation and the subsequent division. These unusual findings suggest that this method for abrupt change in growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell.
First Page
8152
Last Page
8155
DOI
10.1128/jb.174.24
Publication Date
10-2-1992
Recommended Citation
Zaritsky, A., & Helmstetter, C. E. (1992). Rate maintenance of cell division in escherichia coli B/r: Analysis of a simple nutritional shift-down. Journal of Bacteriology, 174(24), 8152-8155.