Document Type
Article
Publication Title
Analytical Chemistry Research
Abstract
Nitrated indolinyl photoprotecting groups are crucial tools extensively used in the study of neuronal signal transduction. Mononitrated photolabile protecting groups have been used effectively, however, recent advances in the introduction of a second nitro group have shown improvement in the photo efficiency of neurotransmitter (agonist) release, albeit, to varying extents, depending on the assessment methods employed. An unambiguous method is discussed based on Nuclear Magnetic Resonance (NMR), which is shown to be an effective technique in the relative overall rate comparison amongst varying nitrated protecting groups. Mononitrated and dinitrated photolabile protecting groups such as CDNIGlu and MNI-Glu are used as an example to assess the relative value of adding a second nitro group in photoactive cage designs. Using this technique, it was shown that the second nitro group in CDNI systems enhances the overall relative rate of photocleavage by a factor of 5.8. This reported method can also be used to unambiguously determine relative rate of agonist photorelease.
First Page
20
Last Page
25
DOI
10.1016/j.ancr.2014.11.001
Publication Date
3-2015
Recommended Citation
Comitz, R. L., Ouedraogo, Y. P., & Nesnas, N. (2015). Unambiguous evaluation of the relative photolysis rates of nitro indolinyl protecting groups critical for brain network studies. Analytical Chemistry Research, 3, 20-25