Date of Award
12-2024
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biomedical Engineering and Sciences
First Advisor
Timothy A. Crombie
Second Advisor
Melissa Borgen
Third Advisor
Robert van Woesik
Fourth Advisor
Linxia Gu
Abstract
Nearly all organisms experience heterogeneity in environmental conditions that can negatively impact fitness. A common adaptive strategy to reduce the impact of this stress is called facultative diapause, where development and reproduction are temporarily arrested to promote survival. Facultative diapause is characterized by three major criteria, the arrest must be (i) induced by an unfavorable environmental condition, (ii) reversible, and (iii) driven by genetically regulated mechanisms. The aim of this thesis was to determine whether chronic heat-induced inhibition of egg laying in Caenorhabditis elegans represents a novel form of facultative diapause. It was found that the inhibition of egg-laying is rapidly induced after 16 hours of heat stress, and is reversible upon return to control conditions for exposures extending up to 24 hours. Unlike another form of reproductive diapause in C. elegans, this work showed that the cessation of egg-laying is not regulated by the gene nhr-49. Heat-induced damage was also investigated as a potential driver of the shutdown. Multiple cellular damage phenotypes were observed in the gonad prior to and immediately after shutdown, with mixed reversibility during recovery. Notably, most phenotypes observed after shutdown do not reverse alongside arrest, potentially indicating a purely correlative relationship. Overall, it remains unclear whether genetic factors, cellular damage, or a combination of both drive the inhibition of egg-laying during chronic heat stress in C. elegans. Further studies will determine whether the shutdown represents a novel form of reproductive diapause, and may ultimately lead to a better understanding of how organisms navigate environmental stress.
Recommended Citation
Nielander, Macy Makenna, "Investigation of Reproductive Inhibition during Chronic Heat Stress in C. elegans" (2024). Theses and Dissertations. 1508.
https://repository.fit.edu/etd/1508
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